Download A Laboratory Guide to the Mammalian Embryo by David K. Gardner, Michelle Lane, Andrew J. Watson PDF

By David K. Gardner, Michelle Lane, Andrew J. Watson

This publication pulls jointly the whole variety of telephone tradition, biochemical, microscopic, and genetic innovations to review the early mammalian embryo. in the past, there hasn't ever been this type of complete compendium, although there were extra centred books of protocol, resembling Manipulating the Mouse Embryo, from chilly Spring Harbor. This ebook is meant to attract all constituencies, from uncomplicated experimental technology to scientific and animal technology functions.

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13, 70. 19. Eppig, J. , and Wigglesworth, K. (1996). Mol. Reprod. , 44, 260. 20. Champlin, A. , Dorr, D. , and Gates, A. H. (1973). Biol. , 8, 491. 3 DAVID K. GARDNER MICHELLE LANE Culture of the Mammalian Preimplantation Embryo 1. EMBRYO CULTURE MEDIA There are several treatises on the composition of embryo culture media and the role of specific medium components in supporting mammalian embryo development in vitro (1–3). It is the aim of this chapter to describe in detail how such media should be used in the laboratory and to identify the potential pitfalls that can lead to impaired embryo development in culture.

Embryos are now ready to be placed into culture. Embryos from some strains are very susceptible to their environment. Therefore, it is important to minimize the time from extraction of the cumulus masses and placement in culture. It is advisable to keep this time period to a maximum of 10 min. Therefore, it is preferable to do multiple smaller embryo collections rather than one large collection. 1. Collection of mouse zygotes from the ampullary region of the oviduct: (a) The swollen ampulla containing the cumulus-oocyte complex is located.

20, 307. Greenwald, G. , and Moor, R. M. (1989). J. Reprod. , 87, 561. , and Winston, R. M. (1999). Hum. , 14, 2519. Wright, C. , Winston, R. , and Hardy, K. (1999). Hum. , 14, 1555. , and Tuuri, T. (2001). Mol. Hum. , 6, 694. Hovatta, O. (2001). Mol. , 169, 95. , and Gosden, R. G. (1998). Fertil. , 69, 1. Picton, H. , and Gosden, R. G. (2000). Mol. , 166, 27. , Hsu, S. , Chun, S. , and Hsueh, A. J. (1997). Endocrinology, 138, 2417. FOLLICLE DEVELOPMENT IN VITRO 23 28. McGee, E. , Nachtigal, M.

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