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RNAi AND FLOWERING TIME Flowering time is one of the important aspects of crop production. As a examples, if flowers of creal crop appear too early, it may have not yet made sufficient energy stores to fuel its maximum grain production. Similarly, in case of late flowering there may be chances of low yield. In Arabidopsis, there is a gene called FLC which represses flowering and we have used RNAi to switch it off and bring on flowering. This clearly shows that the technology has the potential to regulate flowering time in crops .
Due to this, improvement and further research into the kinds of vectors that can be used safely and reliably is needed. There have also been some reports that it has been difficult to detect a mutant in which there has been subtle changes in gene expression . VECTORS USED IN RNAi BASED FUNCTIONAL GENOMICS There are currently many different vectors in use for performing RNAi for the use of functional genomics. These include such vectors as binary vectors used for expression of GUS and GFP proteins, the pHELLSGATE high-throughput gene silencing vector and a high throughput tobacco rattle virus (TRV) based Virus-induced gene silencing (VIGS) vector.
A single copy of a virus derived transgene encoding hairpin RNA gives immunity to barley yellow dwarf virus. Mol Plant Pathol 2000; 1: 401-10. Matthew L. RNAi for plant functional genomics, Comp Funct Genom2004; 5, 240-244 Waterhouse PM , Helliwell CA. Exploring Plant Genomes by RNA-induced Gene Silencing, Nature Reviews: Genetics 2003; 29-38 Agami R. RNAi and related mechanisms and their potential use for therapy. Curr Opin Chem Biol 2002; 6: 829-4. Couzin J. RNA interference. Mini RNA molecules shield mouse liver from hepatitis.