By George Britton, Synnove Liaaen-Jensen, Hanspeter Pfander
The Carotenoids book sequence presents distinct bills of the elemental chemistry of carotenoids and the fundamental equipment utilized in carotenoid examine, and demanding discussions of the biochemistry, capabilities and purposes of those very important compounds.
The ultimate quantity, Volume 5, bargains with the subject that's of maximum common curiosity and public challenge, carotenoids in human wellbeing and fitness and meals. this can be the period of ‘functional foods’, and deciding on roles of chemical elements of meals as vital micronutrients. Carotenoids characteristic excessive at the record of those. Volume 5 follows the carotenoid tale from foodstuff to organic activities.
Carotenoids, quantity 5 is deliberate as a coordinated, built-in therapy delivering updated and demanding learn surveys through prime gurus within the box, and incorporating a few historical past fabric to aid make the chapters obtainable to carotenoid researchers who're now not experts at the specific topic.
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Additional resources for Carotenoids: Nutrition and Health
When isocratic elution is used, the solvent composition is optimized to give the best resolution of the components of interest. Analysis of Carotenoids in Nutritional Studies 15 Polarity, due to the possession of polar functional groups, is the main determinant of interactions between carotenoids and the polar silanol Si-OH groups of the silica stationary phase. Polar groups or regions of the carotenoid molecule compete with polar solvent modifiers for adsorption sites on the stationary phase. The carotenes, with no polar substituents and overall low polarity, have little affinity for normal phase columns and are eluted almost immediately, so selectivity and resolution are not good.
For carotenoids containing oxygen functions (xanthophylls), interactions between polar substituents and the polar stationary phase are the key determinants of chromatographic behaviour. The strongest interactions are with OH or COOH groups. Thus, for example, adsorption affinities and hence retention times increase in the order monohydroxy → dihydroxy → trihydroxy. Additional polar groups further increase the adsorption affinity. Thus xanthophyll epoxides are retained more strongly than the corresponding simple hydroxycarotenoids.
Analysis of Carotenoids in Nutritional Studies 19 b) C30 columns A few years ago, a reversed phase column with a bonded C30 chain stationary phase, prepared by polymeric synthesis, was introduced and has become increasingly popular for the analysis of carotenoids . The selectivity is high and very good resolution of carotenes can be achieved. Molecular shape seems to be a major factor in determining selectivity, and geometrical isomers are well resolved. The high degree of resolution makes the C30 column the one of choice for HPLC-MS of carotenoids.