By Abdelhamid Elaissari
Testifying to the famous versatility of colloidal polymers and heightening estimations in their strength in bioscience functions, Colloidal Biomolecules, Biomaterials, and Biomedical purposes is an authoritative presentation of validated and newfound strategies promising to revolutionize the components of biomedical diagnostics, therapeutics, pharmaceutics, and drug supply.
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Additional resources for Colloidal Biomolecules, Biomaterials, and Biomedical Applications (Surfactant Science)
NUCLEIC ACID SEPARATIONS Magnetic separation and isolation of biomolecules always involves interactions between the colloidal particles and the substance to be separated. Interactions in addition to the extremely effective immunological ones have also been explored for separating different biological molecules. Most have been applied to the separation of nucleic acids. Examples of applications include the following : Purification and concentration of PCR products (or of single-strand DNA fragments) with biotinylated primers and streptavidin-bearing polymer particles, which are used to detect microorganisms or cellular genes, or to label singlestrand probes Specific purification of single-strand DNA or RNA with magnetic particles bearing oligonucleotides (single-strand DNA or RNA fragments of welldefined sequences) Purification of proteins associated with nucleic acids, such as transcription factors, regulatory genes, and promoters Purification of mRNA with magnetic beads coated with polythymidylic acid for in vitro translation or gene expression Nonspecific concentration of nucleic acid followed by PCR or RT-PCR amplification Specific DNA isolation and extraction to obtain purified nucleic acid without the use of any organic solvent as a precipitating agent is required in biomedical diagnostic tests, for which specificity, rapidity, and ease of handling are incontestably necessary.
The global aggregation rate increases strongly with the HCG concentration because the time corresponding to the transition from domain I to domain II decreases from 42 Stoll et al. FIG. 9 Representation of the reduced mass n/S(t) of aggregates present at the greatest concentration as a function of the concentration of IgM molecules expressed by IU/g IgG-coated latex. 6 to 833 IU/g latex. The determination of the initial slope τ of the aggregate mass distribution [Eq.
Elaissari et al. Accelerated Sedimentation Process Biomedical diagnostics are now targeting the development of basic and easy techniques for disease and bacteria detection. Various investigators [49,50] have described an immunoquantification method that is one illustration of such an application. This method discriminates reacting and nonreacting colloidal particles by whether they sediment or coat the sensitive walls of a special tube. After the biological sample is introduced into a recipient whose walls are coated with a substance (such as antibody) with a specific immunological affinity for the targeted substance, sensitive magnetic particles bearing captured analyte are then added to the sensitive tube with a specific immunological affinity for the captured substance.