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J Biol Chem 1984; 259:6364-6368. 87. Wang T r y , Chandler C J , Halsted CH. Intracellular pteroylpolyglutamate hydrolase from human jejunal mucosa. Isolation and characterization. J Biol Chem 1986; 261~13551-13555. 88. Chandler CJ, Wang T N , Halsted CH. Pteroylpolyglutamate hydrolase from human jejunal brush borders. Purification and characterization. JBiolChem 1986; 261~928-933. 89. Whitehead V M , Kalman TI, Vuchich M-J. Inhibition of gamma-glutamyl hydrolases in human cells by2-mercaptomethylglutaricacid.

Folates and Pterins. Vol. 3. New York John Wiley & Sons, 1986: 177-205. 22 Shane 94. Williams W M , Huang KC. Renal tubular transport of folic acid and methotrexate in the monkey. Am J Physioll982; 242:F484-F490. 95. Selhub J, Emmanouel D, Stavropoulos T, Arnold R. Renal folate absorption and the kidney folate binding protein. Am J Physiol 1987; 2525'504756. 96. Eisenga BH, Collins TD, McMartin KE. Effect of acute ethanol on urinary excretion of 5-methyltetrahydrofolic acidandfolate derivatives inthe rat.

Will be discussed below, the methyl group of methionineis metabolically labile. requires It a form of B,, (cyanocobalamin) as a cofactor. During the reaction the methyl group is transferred first from 5methyl-THF to the cofactor to form methylcyanocobalamin, and the methyl group is then transferred to homocysteine [29,30]. C. Enzymes That lnterconvert One€arbon Units Within the Pool The interconversion of 10-formyl-THF and 5,lO-methenyl-THF (reaction 4) is catalyzed by methenyl-THF cyclohydrolase.

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